ID 原文 译文
907 核酸检测是诊断SARS-CoV-2感染的首选方法。 Nucleic acid testing is the preferred method for diagnosing SARS-CoV-2 infection.
908 根据试剂盒说明书进行，一般过程：痰标本等经过前处理，并裂解病毒提取核酸，再用实时（real-time）荧光定量PCR技术扩增SARS-CoV-2的3个特异性基因：开放读码框架1a/b（ORF1a/b）、核壳蛋白 （N）及包膜蛋白（E）基因，检测扩增后荧光强度获得结果。 The testing process according to the kit instructions is as follows: Specimens are pre-processed, and the virus is lysed to extract nucleic acids. The three specific genes of SARS-CoV-2, namely the Open Reading Frame 1a/b (ORF1a/b), nucleocapsid protein (N), and envelope protein (E) genes, are then amplified by real-time quantitative PCR technology. The amplified genes are detected by fluorescence intensity.
909 核酸阳性判断标准： Criteria of positive nucleic acid result s are:
910 ORF1a/b基因阳性，和/或N基因、E基因阳性。 ORF1a/b gene is positive, and/or N gene/E gene are positive.
911 多种类型标本联合检测核酸有利于提高诊断阳性率。 The combined detection of nucleic acids from multiple types of specimens can improve the diagnostic accuracy.
912 在呼吸道标本核酸阳性的确诊患者中，约30%-40%的患者可在其血液中检测到病毒核酸；约50%-60%的患者可在粪便中检测到病毒核酸；尿液标本核酸检出阳性率很低。 Among patients with confirmed positive nucleic acid in Respiratory tract, about 30% - 40% of these patients have detected viral nucleic acid in the blood and about 50% - 60% of patients have detected viral nucleic acid in feces. However, the positive rate of nucleic acid testing in urine samples is quite low.
913 呼吸道标本、粪便、血液等多种 类型标本联合检测有利于提高疑似病例的诊断灵敏度、患者疗效观察和制定合理的出院后隔离管理措施。 Combined testing with specimens from Respiratory tract, feces, blood and other types of specimens is helpful for improving the diagnostic sensitivity of suspected cases, monitoring treatment efficacy and the management of post-discharge isolation measures.
914 病毒分离培养 Virus Isolation and Culture
915 病毒培养必须在获得资格的生物安全三级（BSL-3）实验室开展。 Virus culture must be per formed in a laboratory with qualified Biosafety Level 3 (BSL-3).
916 简要过程：留取患者痰液、粪便等新鲜标本，接种于Vero-E6细胞进行病毒培养，96h后观察细胞病变效应（CPE），并检测培养液病毒核酸阳性即提示培养成功。 The process is briefly described as follows: Fresh samples of the patient's sputum, feces, etc. are obtained and inoculated on Vero-E6 cell s for virus culture. The cytopathic effect (CPE) is observed after 96 hours. Detection of viral nucleic acid in the culture medium indicates a successful culture.