ID 原文 译文
49349 利用Illumina 二代测序和nanopore三代测序技术，获得了病毒的全基因组序列。 Using Illumina and nanopore sequencing, the whole genome sequences of the virus were acquired.
49350 生物信息学分析表明，新型冠状病毒（nCoV-19)具有冠状病毒家族的典型特征，属于β-冠状病毒。 Bioinformatic analyses indicated that the virus had features typical of the coronavirus family and belonged to the Betacoronavirus 2B lineage.
49351 对nCoV-19的全基因组序列和巳有的其他β-冠状病毒的全基因组序列进行一致性比对后显示，该病毒与蝙蝠携带的SARS样冠状病毒RaTG13株全基因组亲缘关系最近，同源性为96%。 Alignment of the full-length genome sequence of the COVID-19 virus and other available genomes of Betacoronavirus showed the closest relationship was with the bat SARS-like coronavirus strain BatCov RaTG13, identity 96%.
49352 利用人呼吸道上皮细胞、Vero E6、Huh-7等不同细胞系进行了病毒分离。 Virus isolation was conducted with various cell lines, such as human airway epithelial cells, Vero E6, and Huh-7.
49353 接种后96小时观察到细胞病变效应(CPE)。 Cytopathic effects (CPE) were observed 96 hours after inoculation.
49354 负染后在透射电子显微镜（TEM)下能观察到典型的冠状颗粒。 Typical crown-like particles were observed under transmission electron microscope (TEM) with negative staining.
49355 从恢复期患者中釆集的血清可以完全中和分离病毒的细胞感染性。 The cellular infectivity of the isolated viruses could be completely neutralized by the sera collected from convalescent patients.
49356 转人ACE2基因小鼠和恒河猴经鼻感染该病毒后，可诱发多灶性肺炎伴间质增生。 Transgenic human ACE2 mice and Rhesus monkey intranasally challenged by this virus isolate induced multifocal pneumonia with interstitial hyperplasia.
49357 随后可在受试动物的肺和肠道组织中检测并分离出该新型冠状病毒。 The COVID-19 virus was subsequently detected and isolated in the lung and intestinal tissues of the challenged animals.
49358 对2019年12月底至2020年2月中旬在不同地点釆集的患者标本中分离出的104株COVID-19病毒株，进行了全基因组测序分析，结果显示它们具有99.9%的同源性，无明显基因突变（图1)。 Whole genome sequencing analysis of 104 strains of the COVID-19 virus isolated from patients in different localities with symptom onset between the end of December 2019 and mid-February 2020 showed 99.9% homology, without significant mutation (Figure 1).